The NT-proBNP test kit is an overnight, 96-well competitive enzyme immunoassay (EIA). This NT-proBNP assay utilizes one antibody that binds to the amino acid region of 8-29 of the NT-proBNP peptide. Thus, the BNP fragment immunoassay is for the quantitative determination of NT-proBNP (1-76), including all NT-proBNP derived fragments containing the amino acid sequence between AA 8-29, in human serum and plasma.