产品概述

The anti-proAKAP4 clone 6F12 recognizes the prodomain of A-kinase anchor protein 4 (AKAP4). This antibody is suitable for Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. This antibody was produced from a mouse hybridoma resulting from a mouse immunized with a peptide covering the prodomain of human AKAP4 protein sequence (Uniprot ref. Q5JQC9) which is 70% homologous between mammals. The anti-pro AKAP4 (clone 6F12) antibody epitope is 100% homologous between mammals. Determined by its ability to recognise the prodomain of human AKAP4 protein. This monoclonal antibody (clone 6F12) only recognizes the pro-form of AKAP4 (110 kDa / 854 AA), the prodomain (21 kDa) and does not react with the AKAP4 (82 kDa / 665 AA). It reacts also with AKAP4 proteins from horse, bull, mouse, rat and ram semens.Human AKAP4 (A-Kinase Anchor Protein 4) protein is encoded by a single gene located on chromosome X. The proAKAP4 (854 amino acids) is processed into mature AKAP4 by proteolytic cleavage of the amino-terminal prodomain made of 188 amino acids. AKAP4 and its precursor proAKAP4, are major components of the pig, horse, bull, mouse, rat, ram, dog, rabbit and human sperm fibrous sheath of the sperm flagellum. AKAP4 protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell. AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase A Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82. AKAP4 plays a major role in sperm motility and capacitation (Miki et al. 2002), that are both essential for oocyte fertilization.