产品概述

The anti-AKAP4 mouse monoclonal antibody clone 7E10 recognizes the carboxy-terminal region of A-kinase anchor protein 4 (AKAP4). This antibody is suitable for Western Blot, for Immunohistochemistry, for ELISA, for Electron Microscopy, for Flow cytometry and Immunofluorescence. This monoclonal antibody was produced from a mouse hybridoma resulting from a mouse immunized with a peptide covering the C-terminus of human AKAP4 protein sequence (Uniprot ref. Q5JQC9) which is 90% homologous between mammals. This antibody is determined by its ability to recognise the C-terminus human AKAP4 protein. The monoclonal antibody (clone 7E10) recognizes both the pro-form of AKAP4 (110 kDa / 854 AA) and the AKAP4 (82 kDa / 665 AA) but does not react with the prodomain (21 kDa). It reacts also with AKAP4 proteins from horse, bull, mouse, rat and ram semens.Human AKAP4 (A-Kinase Anchor Protein 4) protein is encoded by a single gene located on chromosome X. The proAKAP4 (854 amino acids) is processed into mature AKAP4 by proteolytic cleavage of the amino-terminal prodomain made of 188 amino acids. AKAP4 and its precursor proAKAP4, are major components of the pig, horse, bull, mouse, rat, ram, dog, rabbit and human sperm fibrous sheath of the sperm flagellum. AKAP4 protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell. AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase A Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82. AKAP4 plays a major role in sperm motility and capacitation (Miki et al. 2002), that are both essential for oocyte fertilization.