Recommended concentration of use are：
WB：1:4000 in Tris HCl pH 8.0 Tween-20 0.05% and 5% dry skimmed milk (working with cell and tissue)
IHC：1:200 in PBS with 5% BSA (working with cell, frozen and paraffin embedded tissue)
IF：1:200 in PBS with 5% BSA (working with cell, frozen and paraffin embedded tissue)
While the final concentration of use depends on you
Note：WB=Western Blot，IHC=Immunohistochemistry，IF= Immunofluorescence，FCM=Flow cytometry，EM=Electron Microscopy，ELISA=Enzyme Linked Immunosorbent Assay
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use ！
