Stock solutions preparation
1. Dissolve COL G in 900 μl sterile H2O and make 3 aliquots of 300 μl (each aliquot is the Solution A) and store at -20°C 2. Dissolve COL H in 300 μl sterile H2O and make 3 aliquots of 100 μl (each aliquot is the Solution B) and store at -20°C
3. Dissolve the Thermolysin in 150 μl sterile H2O and make 3 aliquots of 50 μl (each aliquot is the Solution C) and store at -20°C.
