产品应用
Biological Activity：Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately >2.5x 107 IU/mg.
SDS-PAGE analysis of recombinant mouse TNF alpha
Note：重悬液进一步稀释分装时，须添加一定量的载体蛋白（0.1%BSA、10%FBS或5%HSA），无血清实验需求时，可更换为5%海藻糖溶液作为载体.
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use ！
- 应用：细胞培养
- 生物学活性：Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately >2.5x 107 IU/mg.
- 内毒素水平：<0.1 EU per 1 μg of the protein by the LAL method.
- 产品组成：N/A
- 产地：台湾
- 存储：-20℃冻干粉长期保存；重悬后的溶液可进一步稀释分装，置于2-8℃有效期1个月，-20℃至-80℃有效期3个月；避免反复冻融.
- 来源：Escherichia coli
- 纯度：>98% as determined by SDS-PAGE. Ni-NTA chromatography.
- 氨基酸序列：MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEGDQLSAEVNLPKYLDFAESGQVYFGVIAL with polyhistidine tag at the C-terminus
- 复溶：推荐使用无菌水重悬冻干粉，溶液浓度不低于100μg/mL，并静置至少20min以充分溶解.
- 运输：常温/蓝冰
