产品应用
Biological Activity:Measure by its ability to induce proliferation in NFS-60 cells.The ED50 for this effect is <50 pg/mL.The specific activity of recombinant human G-CSF is > 2 x 107 IU/mg.
SDS-PAGE analysis of recombinant human G-CSF
Note:重悬液进一步稀释分装时,须添加一定量的载体蛋白(0.1%BSA、10%FBS或5%HSA),无血清实验需求时,可更换为5%海藻糖溶液作为载体.
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use !
- 应用:细胞培养
- 生物学活性:Measure by its ability to induce proliferation in NFS-60 cells.The ED50 for this effect is <50 pg/mL.The specific activity of recombinant human G-CSF is > 2 x 107 IU/mg.
- 内毒素水平:<0.1 EU per 1 μg of the protein by the LAL method.
- 产品组成:从含有1X PBS,pH 7.4 溶液中冻干的蛋白质.
- 产地:台湾
- 存储:-20℃冻干粉长期保存;重悬后的溶液可进一步稀释分装,置于2-8℃有效期1个月,-20℃至-80℃有效期3个月;避免反复冻融.
- 来源:Escherichia coli
- 纯度:>98% as determined by SDS-PAGE. Ni-NTA chromatography.
- 氨基酸序列:TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP with polyhistidine tag at the N-terminus.
- 复溶:推荐使用无菌水重悬冻干粉,溶液浓度不低于100μg/mL,并静置至少20min以充分溶解.
- 运输:常温/蓝冰